Assessment of Allelopathic Activity of Arachis pintoi Krapov. & W.C. Greg as a Potential Source of Natural Herbicide for Paddy Rice

Pinto peanut (Arachis pintoi Krapov. & W.C. Greg.) is an herbaceous perennial plant which belongs to the Leguminosae family. This well known for its use as a cover crop, but little information available on allelopathic potential of this legume. Therefore, study aimed explore effects A. under various screening conditions and analyze allelochemicals using gas chromatography–mass spectrometry (GC-MS). In laboratory bioassays, aqueous extracts powder exerted average inhibition growth Echinochloa crus-galli (55.1%), Oryza sativa (77.1%), Vigna radiata (60.1%), respectively, root lengths tested plants were most suppressed. greenhouse experiments, E. was inhibited by 63.4% at 200 g/m2 dried application. field trials, also significantly reduced natural weeds. By incorporating dose g/m2, 43.9%, dry weight paddy weeds 43.1%, rice yield simultaneously increased 35%. The inhibitions proportional doses applied varied with species. Among three different solvent (methanol, hexane, ethyl acetate), hexane extract highest suppression against L. IC50 shoot = 4.08 8.4 mg/mL 1.7 1.54 mg/mL, followed acetate extract, while least effective methanol extract. From those extracts, total 35 substances detected GC-MS analyses, including 14 newly identified constituents, such phenolic acids, stearic acid, palmitic fatty pyranones, benzofurans, may be responsible herbicidal pintoi. suggests that used source bioherbicide minimize dependency harmful synthetic herbicides enhance yield.